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Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy

DOI :

10.3791/61154-v

May 12th, 2020

May 12th, 2020

7,506 Views

1Pennsylvania Muscle Institute, Perelman School of Medicine, University of Pennsylvania, 2Department of Physics, West Chester University

Described here is a nanosphere lithography method for parallel fabrication of zero mode waveguides, which are arrays of nanoapertures in a metal-clad glass microscopy coverslip for single molecule imaging at nano- to micromolar concentrations of fluorophores. The method takes advantage of colloidal crystal self-assembly to create a waveguide template.

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Zero Mode Waveguides

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