JoVE Logo
Faculty Resource Center

Sign In

Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis

DOI :

10.3791/61180-v

June 18th, 2020

June 18th, 2020

6,553 Views

1Department of Medicine, Monash University, 2Monash Micro Imaging, Monash University, 3Immunology Department, Monash Health

Extracellular DNA (ecDNA) released during cell death is proinflammatory and contributes to inflammation. Measurement of ecDNA at the site of injury can determine the efficacy of therapeutic treatment in the target organ. This protocol describes the use of a machine learning tool to automate measurement of ecDNA in kidney tissue.

Tags

Supervised Machine Learning

-- Views

Related Videos

article

Neutrophil Extracellular Traps: How to Generate and Visualize Them

article

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

article

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

article

Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages

article

High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro

article

Ratiometric Imaging of Extracellular pH in Dental Biofilms

article

Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps

article

A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release

article

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

article

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved