Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

5.7K Views

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08:53 min

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December 4th, 2020

DOI :

10.3791/61967-v

December 4th, 2020

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Valerie P. O'Brien¹^,²^,⁶, Matthew S. Joens³^,⁷, Amanda L. Lewis¹^,²^,⁴^,⁸, Nicole M. Gilbert²^,⁵

¹Department of Molecular Microbiology, Washington University School of Medicine in Saint Louis, ²Center for Women’s Infectious Disease Research, Washington University School of Medicine in Saint Louis, ³Center for Cellular Imaging, Washington University School of Medicine in Saint Louis, ⁴Department of Obstetrics and Gynecology, Washington University School of Medicine in Saint Louis, ⁵Department of Pediatrics, Washington University School of Medicine in Saint Louis, ⁶Fred Hutchinson Cancer Research Center, ⁷TESCAN USA, Inc., ⁸University of California San Diego

Summary

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Recurrent Urinary Tract Infection

Escherichia Coli

Gardnerella Vaginalis

Bladder Exposure

Urogenital Bacteria

Anaerobic Chamber

Culture Incubation

OD600 Measurement

Centrifuge Procedures

Epithelial Cells

Fixative Syringe

Catheterization Technique

Chapters in this video

0:05

Introduction

0:41

Bladder Exposures to G. vaginalis

1:53

Urine Cytology

2:31

Imaging Bladders by Scanning Electron Microscopy

6:23

Results: G. vaginalis Triggers UPEC rUTI

8:27

Conclusion

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