Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

4.9K Views

07:27 min

April 9th, 2021

DOI :

10.3791/62107-v

April 9th, 2021


Transcript

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Alkynyl Fatty Acid Analogs

Chapters in this video

0:04

Introduction

0:44

Cell Culture

1:25

Preparation and Saponification of Fatty Acid Analogs

3:35

Click Reaction on Cell Lysates

4:12

Click Reaction on Immunoprecipitated Proteins

5:13

Results: Detection of Fatty Acylated Proteins Using Click Chemistry

6:57

Conclusion

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