Titration of His-Tagged Proteins on 12.5 mol% of DGS-(Ni) NTA-Containing BSLBs
4:02
Measurement of T-cell Output Transfer to BSLBs
8:13
Results: FCM for Absolute Protein Quantification on the Cell Surface
10:17
Conclusion
Transcript
The study of the output of the immune synapse has been restricted mostly to microscopic methods. BSLBs have expanded our repertoire of tools that can be used to study the output of T cell immune synapses, including flow cytometry, microscopy, prot
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Here we present the protocol for the stepwise reconstitution of synthetic antigen-presenting cells using Bead-Supported Lipid Bilayers and their use to interrogate the synaptic output from activated T cells.