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JoVE Journal

Medicine

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In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

1.5K Views

09:17 min

September 7th, 2022

September 7th, 2022

1,451 Views

0:05

Introduction

0:35

Anesthesia and Ganglionic Block

1:29

Surgical Procedure: Suprapubic Bladder Catheterization

2:29

Transurethral Catheterization

3:43

Experimental Setup

5:08

Collection of Samples

6:20

Quantifying ATP from Collected Samples

7:03

Results: Measurement of Urothelial ATP Release In Vivo from the Lumen of the Bladder

8:39

Conclusion

Transcript

Our protocol measures ATP, an important transmitter in the urinary bladder, which will allow us to further interrogate the mechanisms controlling its release. Our technique measures ATP directly from the bladder lumen, making it more physiological

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This protocol describes the procedure for measuring ATP concentrations in the lumen of the bladder in an anesthetized rodent.

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