Development of Leishmania Species Strains with Constitutive Expression of eGFP
681 Views
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10:03 min
•
April 21st, 2023
DOI :
April 21st, 2023
•Jafeth Carrasco*1, Jim H. Chang*1, Laura Pineda1, Iliana Quintero1, Rita Giovani2, Carmenza Spadafora1,3, Ricardo Lleonart1,3, Carlos M. Restrepo1,3
1Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP), 2Departamento de Medios y Creativo, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP), 3Sistema Nacional de Investigación-Secretaría Nacional de Ciencia, Tecnología e Innovación (SNI-SENACYT)
Chapters in this video
0:04
Introduction
0:27
Amplification and Insertion of the eGFP Gene into the pLEXSY‐2.1 Vector
2:39
Linearization of the pLEXSY Expression Plasmid
3:26
Transfection of L. panamensis and L. donovani by Electroporation
5:00
Polyclonal Selection in Culture
6:41
Confirmation of Genomic Integration
7:09
Cloning by Limiting Dilution
8:12
Results: Confirmation of eGFP Cassette Expression and Chromosomal Insertion
9:40
Conclusion
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