677 Views
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10:03 min
•
April 21st, 2023
DOI :
April 21st, 2023
•0:04
Introduction
0:27
Amplification and Insertion of the eGFP Gene into the pLEXSY‐2.1 Vector
2:39
Linearization of the pLEXSY Expression Plasmid
3:26
Transfection of L. panamensis and L. donovani by Electroporation
5:00
Polyclonal Selection in Culture
6:41
Confirmation of Genomic Integration
7:09
Cloning by Limiting Dilution
8:12
Results: Confirmation of eGFP Cassette Expression and Chromosomal Insertion
9:40
Conclusion
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