Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies
747 Views
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10:23 min
•
May 19th, 2023
DOI :
May 19th, 2023
•Juliane Corrêa Glória1,2,3, Yury Oliveira Chaves2,3,4, Adélia Marques de Figueiredo1, Caio Coutinho de Souza2, Eliza Raquel Duarte da Silva2, Jeniffer Clorives Lopes Batista2,5, Paulo Afonso Nogueira2,3,5, Luis André Morais Mariúba1,2,3,5
1Programa de Pós-Graduação em Biotecnologia da Universidade Federal do Amazonas - UFAM, 2Instituto Leônidas e Maria Deane (ILMD), Fundação Oswaldo Cruz (FIOCRUZ), 3Programa de Pós-Graduação em Biologia da Interação Patógeno-Hospedeiro do Instituto Leônidas e Maria Deane (ILMD), Fundação Oswaldo Cruz (FIOCRUZ), 4Programa de Pós-graduação em Ciências Aplicadas à Hematologia – Universidade Estadual do Amazonas/Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas, 5Programa de Pós-Graduação em Imunologia Básica e Aplicada, Instituto de Ciências Biológicas, Universidade Federal do Amazonas (UFAM)
Chapters in this video
0:04
Introduction
0:45
Extraction of IgY from Egg Yolks
2:27
Saturation Curve of IgY Antibodies to Latex Beads
4:02
Flow Cytometry Assay Based on Latex Beads for Antigen Detection
5:05
Flow Cytometer Reading of the Samples and Sample Analysis
7:33
Results: Electrophoretic Profile of the IgY‐PfHRP2 Antibody
9:29
Conclusion
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