CRISPR-Based Modular Assembly for High-Throughput Construction of a UAS-cDNA/ORF Plasmid Library
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05:30 min
•
May 17th, 2024
DOI :
May 17th, 2024
•Si Xu*1,2, Mengyu Chen*1,2, Guang Chen1,3, Si Luo4, Wen Xue1,2, Xuelian Liu1,2, Jiwu Wang1,2,3, Ping Wei4
1Clinical Research Institute, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, 2Department of Neurology, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, 3Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, 4Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
Chapters in this video
0:00
Introduction
1:21
Large‐Scale Construction of UAS‐cDNA/ORF Plasmids by CRISPR‐Based Modular Assembly (CRISPRmass)
3:44
Large‐Scale Transformation of UAS‐cDNA/ORF Plasmids from CRISPR‐Based Modular Assembly into E. coli
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