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Protocols for C-Brick DNA Standard Assembly Using Cpf1

DOI :

10.3791/55775-v

June 15th, 2017

June 15th, 2017

7,963 Views

1Institute of Plant Physiology and Ecology, Shanghai Insititutes for Biological Sciences, Chinese Academy of Sciences

CRISPR-associated protein Cpf1 can be guided by a specially designed CRISPR RNA (crRNA) to cleave double-stranded DNA at desired sites, generating sticky ends. Based on this characteristic, a DNA assembly standard (C-Brick) was established, and a protocol detailing its use is described here.

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