JoVE Logo
Faculty Resource Center

Sign In

Protocols for C-Brick DNA Standard Assembly Using Cpf1

DOI :

10.3791/55775-v

June 15th, 2017

June 15th, 2017

8,027 Views

1Institute of Plant Physiology and Ecology, Shanghai Insititutes for Biological Sciences, Chinese Academy of Sciences

CRISPR-associated protein Cpf1 can be guided by a specially designed CRISPR RNA (crRNA) to cleave double-stranded DNA at desired sites, generating sticky ends. Based on this characteristic, a DNA assembly standard (C-Brick) was established, and a protocol detailing its use is described here.

Tags

Keywords C Brick

-- Views

Related Videos

article

Methyl-binding DNA capture Sequencing for Patient Tissues

article

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

article

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

article

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

article

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

article

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation

article

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

article

Methodology for Accurate Detection of Mitochondrial DNA Methylation

article

A Customizable Protocol for String Assembly gRNA Cloning (STAgR)

article

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved