2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
3.7K Views
•
14:19 min
•
March 25th, 2022
DOI :
March 25th, 2022
•Lucile Boutaud*1, Marie Michael*1, Céline Banal2, Damelys Calderon1, Sarah Farcy1, Julie Pernelle1, Nicolas Goudin3, Camille Maillard1, Clémantine Dimartino1, Cécile Deleschaux1, Sébastien Dupichaud4, Corinne Lebreton1, Sophie Saunier1, Tania Attié-Bitach1,5, Nadia Bahi-Buisson1,6, Nathalie Lefort2, Sophie Thomas1
1Imagine Institute, INSERM UMR 1163, Université de Paris, 2Imagine Institute, iPSC Core Facility, INSERM UMR U1163, Université de Paris, 3Necker Bio-image Analysis platform of the SFR Necker, 4Imagine Institute, Cell Imaging Platform, INSERM-US24-CNRS UMS 3633 Structure Fédérative de Recherche Necker, INSERM UMR U1163, Université de Paris, 5Fédération de Génétique, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Pediatric Neurology, APHP- Necker Enfants Malades Hospital
Explore More Videos
Chapters in this video
0:07
Introduction
1:42
Generation of 2D hIPS Cell-Based Models of Neocortical Development
2:56
Generation of Dorsal Forebrain Organoids
4:56
In toto Immunolabeling, Clearing, and Lightsheet Acquisition of Dorsal Forebrain Organoids
7:00
Immunostaining and Clearing of Free-Floating Sections of Dorsal Forebrain Organoids
9:20
Representative Results
13:11
Conclusion
Related Videos
17:28
Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
12.5K Views
10:18
Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
11.4K Views
12:08
Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
11.1K Views
07:51
Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
9.1K Views
10:48
Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
13.2K Views
06:47
Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)
8.7K Views
11:38
RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
10.3K Views
09:54
Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
13.3K Views
06:21
In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
9.3K Views
08:37
Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
5.3K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved