The novel tank assay will combine with powerful genetic tools in Zebrafish offers a method for dissecting how the brain modulates stress and how neural circuits may be disregulated. Genetic and neuronal signaling pathways are highly conserved betw
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This manuscript describes a simple method to measure stress behaviorally in adult zebrafish. The approach takes advantage of the innate tendency that zebrafish prefer the bottom half of a tank when in a stressful state. We also describe methods for coupling the assay with pharmacology.