Our protocol provides and easy and fast approach for primary fibroblast isolation with a clear focus on avoiding bacteria contamination, which can be a huge problem. Especially for beginners. The main advantage of our technique is its simplicity.<
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We present a protocol to isolate primary adult fibroblasts in an easy, fast and reliable way, performable by beginners (e.g., students). The procedure combines enzymatic tissue digestion and mechanical agitation with ultrasonic waves to obtain primary fibroblasts. The protocol can easily be adapted to specific experimental requirements (e.g., human tissue).