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Flow Cytometry-based Purification of S. cerevisiae Zygotes

12.7K Views

15:09 min

September 21st, 2012

September 21st, 2012

12,727 Views

0:05

Title

2:03

Haploid Cell Growth

3:08

Conducting a Cross

4:43

Harvesting Preparations

5:57

Flow Cytometry

9:01

Preparation for DeltaVision Microscopy

11:05

Representative Analysis of Zygote Purification

14:27

Conclusion

To purify zygotes of S. cerevisiae, haploid cells of opposite mating type were engineered to express red or green fluorescent proteins, co-incubated to allow zygote formation, and fractionated using a flow cytometry-based protocol. The highly-enriched fraction enables subsequent "-omic" studies, recovery of initial progeny, and systematic investigation of zygote morphogenesis.

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