Results: Evaluating the Efficiency of CRISPR/Cas9 Gene Editing of HSPCs
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Conclusion
Transkript
Ex vivo culture can hamper the repopulation potential of hematopoietic stem and progenitor cells. Our protocol preserves the stemness during the culture and thus improves the frequency of gene-modified cells in vivo. Hematopoietic stem cell gene t
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The present protocol describes an optimized hematopoietic stem and progenitor cell (HSPC) culture procedure for the robust engraftment of gene-edited cells in vivo.