Preparation of the Diaphragms and Phrenic Nerves from Transgenic Mice
1:27
Stimulation and Recording of the Muscle Action Potentials
4:49
Imaging of the Fluorescence of the Sample
6:33
Results: Nerve Stimulation-induced Ca2+ Responses in Populations of Schwann Cells or Muscle Cells of the Neonatal Mouse Diaphragm
8:03
Conclusion
Transcripción
This method can help answer key questions in the neuromuscular field, such as the role of calcium signaling in muscle or Schwann cells in response to nerve stimulation. The main advantage of this technique is that one can image the activation of s
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Here we present a protocol to image calcium signaling in populations of individual cell types at the murine neuromuscular junction.