Soft Light Lamp Preparation (Day 1), EB Transfer, and Medium Replacement (Days 2 5)
4:24
Neural Induction (Days 5 7)
5:25
Embedding in the Basement Membrane Matrix (Days 7 10)
6:52
Organoid Maturation (Days 10 40)
7:21
Results: Analysis of Cerebral Organoids
8:41
Conclusion
Transcribir
The protocol facilitates operation of cerebral organoids culture and helps to promote the application of brain organoids. The earlier stage organoid can be seen with the naked eye by using a lateral soft light lamp which is convenient for many ope
Inicia sesión o comienza tu prueba gratuita para acceder a este contenido
Cerebral organoids provide unprecedented opportunities for studying organ development and human disease pathology. Although great success has been achieved with cerebral organoid culture systems, there are still operational difficulties in applying this technology. The present protocol describes a cerebral organoid procedure that facilitates medium change and organoid transfer.