Identifying the Cell Viability and Cellular Dendrites Using a Confocal Microscope
6:00
Identifying Appearance Using a Stereomicroscope
6:55
Identifying Mineral Deposition by XRF Assay
7:45
Identifying Functional Gene Expression
8:25
Results: Dendrite Network, Mineral Deposition, and Gene Expression of Osteogenic Cultured IDG-SW3 Cells
10:04
Conclusion
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We detailed a protocol to establish a 3D system using an extracellular metrics along with collagen one for analyzing formation and gene expression through osteogenesis. Extracellular metrics can increase the transparency of the collagen one form g
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Here, we present a protocol for culturing IDG-SW3 cells in a three-dimensional (3D) extracellular matrix.