Endothelial-Cell-Enriched Single-Cell Isolation from Mice Carotids
4:02
Flushing the Carotid Arteries
5:53
Single-Cell and Single-Nucleus Analyses
7:18
Results: Standard QC Metrics for the scRNAseq and scATACseq Study
8:34
Conclusion
Transcription
In a blood vessel, the number of endothelial cells is very low, which makes it difficult to study them. Our protocol eliminates this limitation, and allows us to study novel molecular pathways and therapeutics. Our protocol allows us to obtain end
Connectez-vous ou commencez votre essai gratuit pour accéder à ce contenu.
We present a method for isolating endothelial cells and nuclei from the lumen of mouse carotid arteries exposed to stable or disturbed flow conditions to perform single-cell omics experiments.