Preparation of 1.5% Agarose Gel for Electrophoresis and DNA Band Detection
4:22
Results: Genotype Analysis and Recombination Efficiency
5:21
Conclusion
Transcription
The difficulty with a single residue substitution is to genotyping animals efficiently despite the difference in single residue. Special use of a restriction enzyme adds only a single, rapid, and low-cost step to typical PCR-based genotyping. This
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The present protocol describes a single M213L mutation in Gja1 that retains full-length Connexin43 generation but prevents translation of the smaller GJA1-20k internally translated isoform.