Isolation of Bone Marrow Progenitors from Tibia and Femur
3:07
Macrophage Culture
3:57
Harvesting Macrophages
5:39
Results: Characterizing the Bone Marrow-Derived Macrophages
6:42
Conclusion
Transcription
This protocol aims to obtain high amounts of unstimulated macrophages that have been derived from bone marrow and have never been exposed to tissue factors or cytokines. The main advantage of this technique is the number of cells acquired from a s
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The present protocol describes the isolation and culture of bone marrow-derived macrophages from mice.