Characterizing gene expression at the transcriptomic level is key to understanding cell function in health and disease. Our method focuses on capturing endothelial cells, the crucial layer of the vascular wall, from human donors'menesteric arterie
Accedi o inizia la tua prova gratuita per vedere questo contenuto
The protocol describes the isolation, culture, and profiling of endothelial cells from human mesenteric artery. Additionally, a method is provided to prepare human artery for spatial transcriptomics. Proteomics, transcriptomics, and functional assays can be performed on isolated cells. This protocol can be repurposed for any medium- or large-size artery.