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Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity

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07:15 min

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November 22nd, 2021

DOI :

10.3791/62626-v

November 22nd, 2021

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2,039 Views

Xiaoyan Chang*¹, Keru Zhou*¹, Yanguang Liu¹^,², Lian Duan³, Lichun Zhang², Guoliang Zhang³, Haikun Wang⁴, Guiqin Wang¹

¹Nanjing Advanced Academy of Life and Health, ²Institute of Animal Bio-technology, Jilin Academy of Agricultural Sciences, ³National Clinical Research Center for Infectious Diseases, Shenzhen Third People’s Hospital, Southern University of Science and Technology, ⁴CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences

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Pseudo typed H5 Avian Influenza Viruses

Calcium Phosphate Transfection

Antibody Neutralizing Activity

Hemagglutinin Protein Expression

Pseudo Virus Titration

MDCK Cells

Luciferase Assay

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