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Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.

DOI :

10.3791/50838-v

November 20th, 2013

November 20th, 2013

13,486 Views

1Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, 2IKERBASQUE, Basque Foundation for Science

Drosophila S2 cells and cultured neurons are great systems for imaging of motor-driven organelle transport in vivo. Here we describe detailed protocols for culturing both cell types, their imaging and analysis of transport.

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Keywords Drosophila

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