Blastocyst Recording During Equilibration, Cross-Sectional Area Measurement, Data Editing and Creation of a Line Diagram
3:27
Blastocyst Time-Lapse Re-Expansion Recording, Measurements, Data Editing and Creation of Line Diagram
6:09
Results: Representative Blastocoel Contraction and Re-Expansion Analyses
7:49
Conclusion
필기록
This morphometric protocol allows monitoring of the intensity of blastocyst shrinkage and re-expansion during prior vitrification interventions and post-warming recovery and provides insights into the effectiveness of virus vitrification methods.
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Here we present a time-lapse morphometric protocol to follow the intensity of blastocyst shrinkage and re-expansion during previtrification interventions and post-warming recovery. The application of the protocol is possible in in vitro fertilization laboratories equipped with time-lapse microscopes and is recommended in the development of an optimal blastocyst vitrification method.