Bone Marrow, Liver, Thymus-Humanized (hu-BLT) Mouse Generation
3:43
Human Immune Cell Level Evaluation and Fresh Fecal Sample Collection
5:20
Fecal Transplant
6:05
Results: Representative Double Humanized BLT-Mouse Gut Microbiome Analyses
7:02
Conclusion
필기록
Our protocol is significant, because it can be use to reproducibly create mice with both a human gut microbiome and a human immune system. We call these mice, double humanized mice. The double humanized mouse model can be used for both basic and t
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
We describe a novel method for generating double humanized BLT-mice that feature a functional human immune system and a stable engrafted human-like gut microbiome. This protocol can be followed without the need for germ-free mice or gnotobiotic facilities.