October 27th, 2019
•Here, we describe a single cell micro-aspiration method for the separation of infected amoebae. In order to separate viral subpopulations in Vermamoeba vermiformis infected by Faustoviruses and unknown giant viruses, we developed the protocol detailed below and demonstrated its ability to separate two low-abundance novel giant viruses.
Tags
Related Videos
Fluorescence Activated Cell Sorting of Plant Protoplasts
Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton
Agarose Gel Electrophoresis for the Separation of DNA Fragments
Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting
Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
Isolation of Normal and Cancer-associated Fibroblasts from Fresh Tissues by Fluorescence Activated Cell Sorting (FACS)
The MultiBac Protein Complex Production Platform at the EMBL
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
An Alternative Approach for Sample Preparation with Low Cell Number for TEM Analysis
Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation
Copyright © 2024 MyJoVE Corporation. 판권 소유