Polysome Purification from Soybean Symbiotic Nodules
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07:02 min
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July 1st, 2022
DOI :
July 1st, 2022
•María Martha Sainz1, Carla Valeria Filippi1, Guillermo Eastman2,3, Mariana Sotelo-Silveira1, C. Mauro Martinez1, Omar Borsani1, José Sotelo-Silveira2,4
1Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República, 2Departamento de Genómica, Instituto de Investigaciones Biológicas Clemente Estable, 3Department of Biology, University of Virginia, 4Departamento de Biología Celular y Molecular, Facultad de Ciencias, Universidad de la República
Rozdziały w tym wideo
0:05
Introduction
0:36
Preparation of Cytosolic Extracts and Sucrose Cushions
2:09
Polysome Purification
3:05
RNA Extraction, Quality Control, and Precipitation
5:24
Results: Quality Assessment of the TOTAL and PAR Fractions
6:24
Conclusion
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