Preparation of Cytosolic Extracts and Sucrose Cushions
2:09
Polysome Purification
3:05
RNA Extraction, Quality Control, and Precipitation
5:24
Results: Quality Assessment of the TOTAL and PAR Fractions
6:24
Conclusion
Transcription
This protocol is significant because it is, to our knowledge, the first one to describe a new centrifuge based metal for kinetic polysome purification from soybean symbiotic nodules. The big advantage of this technique is that in combination with
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This protocol describes a method for eukaryotic polysome purification from intact soybean nodules. After sequencing, standard pipelines for gene expression analysis can be used to identify differentially expressed genes at the transcriptome and translatome levels.