National Institutes of Health, AR53579, Osteogenesis: Potentiation with Bone Tropic Peptides 
National Institutes of Health, AR056422, Anabolic Effect of Osteogenic Peptides 
National Institutes of Health, T32 AR050960, Musculoskeletal Tissue Repair and Regeneration 
DOD, PC051219 Nucleolin: A Novel Mediator of Prostate Cancer and Bone Marrow Endothelial Cell Adhesion 
Department of Defense, PC061508, Prostate Cancer Metastasis Cell-Bone Marrow adhesion Mediators

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The production of this video was sponsored by New England Biolabs, Inc, which produces some of the reagents used in this article.

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<td>Phage Display Peptide Library Kit-Ph.D-12</td>
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peptides from phage display library modulate gene expression in mesenchymal cells and potentiate osteogenesis in unicortical bone defects

Two novel synthetic peptides accelerate bone formation and can be delivered using a collagen matrix. The aim of this study was to investigate the effects on bone repair in a unicortical defect model. Treatment of mesenchymal cells produced an increase in alkaline phosphatase activity, showed nodule formation by the cells, and increased the expression of genes for runx2, osterix, bone sialoprotein, and osteocalcin. A collagen sponge soaked with peptide promoted repair of bone defects, whereas the control was less effective. The results from this study demonstrated that mesenchymal cells treated with peptide in vitro differentiate towards osteogenesis, and, that peptides delivered in vivo using a collagen sponge promote the repair of unicortical defects.

Watch this Scientific Journal Video about Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects at JoVE.com

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

A phage display library was used to identify peptide sequences that target bone. The objective was to investigate the effect of these peptides on mesenchymal cell differentiation and to determine their effect on bone regeneration.

Two novel synthetic peptides accelerate bone formation and can be delivered using a collagen matrix. The aim of this study was to investigate the effects ...

peptides-from-phage-display-library-modulate-gene-expression

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JoVE Journal

Biology

17.9K visualizações.  University of Virginia. Uma biblioteca de mostrar fago foi utilizado para identificar seqüências de peptídeos que o osso alvo. O objetivo foi investigar o efeito destes peptídeos sobre a diferenciação de células mesenquimais e determinar seus efeitos sobre a regeneração óssea.