Wholemount In Situ Hybridization for Astyanax Embryos

0:40

Day 1: Rehydration

1:30

Day 1: Digestion and Fixation

2:42

Day 1: Prehybridization and Day 2: Hybridization

3:46

Day 3: Solution Preparation and Probe Removal

5:02

Day 3: Blocking

5:42

Day 4: MABT Rinses

6:34

Day 5: Probe Visualization

8:00

Results: Analysis of Mounted Astyanax Embryos

9:37

Conclusion