Establishment of Apical-Out Enteroid Necrotizing Enterocolitis (NEC)-in-a-Dish Model
2:44
Immunofluorescence Staining and Confocal Microscopy
5:52
Apical-Out NEC-in-a-Dish Cell Viability
7:01
Results: Establishment of Apical-Out NEC in-a-Dish Model and Assessing Viability of Enteroids
8:28
Conclusion
Transcrição
This protocol describes the first apical-out in-a-dish model and is a critical improvement over basal lateral out enteroid in-a-dish models when establishing in vitro modeling of potential therapeutics intended for oral administration. This method
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This protocol describes an apical-out necrotizing enterocolitis (NEC)-in-a-dish model utilizing small intestinal enteroids with reversed polarity, allowing access to the apical surface. We provide an immunofluorescent staining protocol to detect NEC-related epithelial disruption and a method to determine the viability of apical-out enteroids subjected to the NEC-in-a-dish protocol.