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Abstract

Bioengineering

4D Light-sheet Imaging of Zebrafish Cardiac Contraction

Published: January 5th, 2024

DOI:

10.3791/66263

1Department of Bioengineering, The University of Texas at Dallas, 2Center for Imaging and Surgical Innovation, The University of Texas at Dallas, 3Hamon Center for Regenerative Science and Medicine, UT Southwestern Medical Center

Abstract

Zebrafish is an intriguing model organism known for its remarkable cardiac regeneration capacity. Studying the contracting heart in vivo is essential for gaining insights into structural and functional changes in response to injuries. However, obtaining high-resolution and high-speed 4-dimensional (4D, 3D spatial + 1D temporal) images of the zebrafish heart to assess cardiac architecture and contractility remains challenging. In this context, an in-house light-sheet microscope (LSM) and customized computational analysis are used to overcome these technical limitations. This strategy, involving LSM system construction, retrospective synchronization, single cell tracking, and user-directed analysis, enables one to investigate the micro-structure and contractile function across the entire heart at the single-cell resolution in the transgenic Tg(myl7:nucGFP) zebrafish larvae. Additionally, we are able to further incorporate microinjection of small molecule compounds to induce cardiac injury in a precise and controlled manner. Overall, this framework allows one to track physiological and pathophysiological changes, as well as the regional mechanics at the single-cell level during cardiac morphogenesis and regeneration.

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Keywords 4D Light sheet Imaging

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