Perfusion, Triple Fluorescent Staining, and Tissue Clearing Treatment
3:10
Results: Enhanced Understanding of the Skin Innervation via Immunofluorescence and a 3D View of the Cutaneous Nerve Fibers in the Hairy and Glabrous Skin
4:31
Conclusion
Transkript
Our protocol provided a feasible method to observe the sync section using confocal microscope, which could ensure precision in the observation of the nerve fibers. Simple and easy tissue cleaning protocol is a main advantage of this technique. The
Bu içeriğe erişmek için giriş yapın veya ücretsiz denemenizi başlatın
The thickness of tissue sections limited the morphological study of the skin innervation. The present protocol describes a unique tissue clearing technique to visualize cutaneous nerve fibers in thick 300 µm tissue sections under confocal microscopy.