Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System
15.9K Views
•
11:47 min
•
August 1st, 2016
DOI :
August 1st, 2016
•Chapters in this video
0:05
Title
0:41
Amino Acid Solution Preparation
3:23
Cell-free Reactions for Residue-specific Incorporation of ncAAs
5:06
SDS-PAGE of Cell-free Reactions
6:57
His-tagged Model Protein Purification from the Cell-free Reactions
8:48
Buffer Exchange for HPLC-ESI Mass Spectroscopy
10:03
Results: Successful Incorporation of ncAA in Target Protein
11:14
Conclusion
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