October 3rd, 2018
•Here we present a protocol for anaerobic protein purification, anaerobic protein concentration, and subsequent kinetic characterization using an oxygen electrode system. The method is illustrated using the enzyme DesB, a dioxygenase enzyme which is more stable and active when purified and stored in an anaerobic environment.
Tags
Related Videos
Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b
Expression, Purification, and Antimicrobial Activity of S100A12
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
Studying Protein Import into Chloroplasts Using Protoplasts
Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments
Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved