April 22nd, 2019
•We describe approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus. Three different techniques are described: Tol2-mediated transgenesis, targeted manipulation of the genome using CRISPR/Cas9, and knockdown of expression using morpholinos. These tools should facilitate the direct investigation of genes underlying the variation between surface- and cave-dwelling forms.
Related Videos
Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning
Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation
Manipulation of Ploidy in Caenorhabditis elegans
Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
Culturing and Manipulation of O9-1 Neural Crest Cells
Raising the Mexican Tetra Astyanax mexicanus for Analysis of Post-larval Phenotypes and Whole-mount Immunohistochemistry
Copyright © 2024 MyJoVE Corporation. 판권 소유