Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens
9.7K Views
•
10:59 min
•
May 12th, 2019
DOI :
May 12th, 2019
•Chapters in this video
0:04
Title
0:38
Differentiation from iPSCs to the Hindgut
1:10
Embedding of the Hindgut in Basement Membrane Matrix
3:13
Maintenance, Passage, and Pre-stimulation of iHOs with rhIL-22
5:28
Microinjection of iHOs and Intracellular Invasion Assays
9:19
Results: Studying Epithelial Cell Protection Against Pathogens Using hiPSC-derived iHOs
10:05
Conclusion
Related Videos
Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
19.4K Views
Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
22.3K Views
Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori
35.1K Views
Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens
8.7K Views
Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
8.6K Views
Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
10.3K Views
Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
8.9K Views
Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens
12.0K Views
Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers
8.5K Views
Chronic Salmonella Infection Induced Intestinal Fibrosis
6.9K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved