Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells

8.5K Views

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10:03 min

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July 5th, 2019

DOI :

10.3791/59696-v

July 5th, 2019

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Michael E. Bregenzer*¹, Ciara Davis*¹, Eric N. Horst*¹, Pooja Mehta*², Caymen M. Novak*¹, Shreya Raghavan*², Catherine S. Snyder*², Geeta Mehta¹^,²^,³^,⁴

¹Department of Biomedical Engineering, University of Michigan, ²Department of Materials Science and Engineering, University of Michigan, ³Rogel Cancer Center, School of Medicine, University of Michigan, ⁴Macromolecular Science and Engineering, University of Michigan

Summary

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3D Spheroids

Anti neoplastic Drug Screening

Cancer Stem Cells

Patient derived Samples

Drug Resistance

Hanging Drop Model

Ovarian Cancer

Chemotherapy Heterogeneity

Targeted Therapies

Cell Culture Methods

Cell Suspension Preparation

Spheroid Maintenance

High Throughput Screening

Chapters in this video

0:04

Title

1:26

Generation of Spheroids from Small Cell Numbers

2:44

Quantification of Proliferation and Viability within Spheroids

3:33

Analysis of Cancer Stem Cell Populations with Flow Cytometry

5:15

FACS Data Analysis

8:03

Results: Patient-derived Cancer Stem Cells within 3D Hanging Drops

9:03

Conclusion

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