Using intravital microscopy, this protocol enables the real-time visualization of intestinal tissue up to a single-cell resolution and the study of highly dynamic processes such as cell shedding. In combination with the standard tracer experiments
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Taking advantage of intravital microscopy, the method presented here enables real-time visualization of intestinal epithelial cell shedding in living animals. Therefore, topically stained intestinal mucosa (acriflavine and rhodamineB-dextran) of anesthetized mice is imaged up to single-cell resolution using confocal microscopy.