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Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases

DOI :

10.3791/61347-v

14:57 min

October 10th, 2020

October 10th, 2020

13,541 Views

1Research platform of Pediatric Onco-Hematology, Department of Paediatrics, Gynaecology and Obstetrics, Faculty of Medicine, University of Geneva, 2Section of Biology, Faculty of Science, University of Geneva, 3Onco-Hematology Unit, Department of Women, Children-Adolescents, University Hospitals of Geneva

Glutathione S-transferases (GSTs) are detoxification enzymes involved in the metabolism of numerous chemotherapeutic drugs. Overexpression of GSTs is correlated with cancer chemotherapy resistance. One way to counter this phenotype is to use inhibitors. This protocol describes a method using a spectrophotometric assay to screen for potential GST inhibitors.

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Keywords Spectrophotometric Screening

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