Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
14.3K Views
•
14:57 min
•
October 10th, 2020
DOI :
October 10th, 2020
•1Research platform of Pediatric Onco-Hematology, Department of Paediatrics, Gynaecology and Obstetrics, Faculty of Medicine, University of Geneva, 2Section of Biology, Faculty of Science, University of Geneva, 3Onco-Hematology Unit, Department of Women, Children-Adolescents, University Hospitals of Geneva
Chapters in this video
0:00
Introduction
0:56
Preparation of the Enzymatic Solution
3:10
Michaelis-Menten Constant of CDNB with GST
4:42
Absorbance of Potential GST Inhibitors
6:08
Determination of the IC50
7:49
Determination of the Type of Inhibition and Ki
9:46
Results: Testing the Potential of Curcumin as a GST Inhibitor
13:56
Conclusion
Related Videos
10:44
Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
9.7K Views
09:33
Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
7.4K Views
08:14
Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans
254 Views
Hellgrammite as a Non-Conventional Biomonitor for Surface Water and Environmental Health Assessment
146 Views
06:07
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
2.5K Views
03:35
Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
1.1K Views
06:17
Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
385 Views
09:22
The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
10.5K Views
09:51
Cell-Based Drug Screening for Inhibitors of Autophagy Related 4B Cysteine Peptidase
714 Views
01:26
Amplified Luminescent Proximity Homogeneous Assay: A Bead-Based Proximity Assay to Screen Small Molecules Inhibiting Protein-Protein Interactions
64 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved