JoVE Logo

Sign In

Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome

3.0K Views

09:39 min

December 4th, 2021

DOI :

10.3791/62240-v

December 4th, 2021


Explore More Videos

Induced Pluripotent Stem Cells

Chapters in this video

0:05

Introduction

1:12

Fibroblast Isolation from Fetal Tissue

2:49

Plasmid Isolation and Verification

3:27

Nucleofection

6:45

iPSC Expansion

7:22

iPSC Characterisation

7:34

Differentiation to Cells of Three Germ Layers

8:38

Results

9:16

Conclusion

Related Videos

article

07:29

Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue

15.4K Views

article

08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

6.9K Views

article

07:40

Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders

19.7K Views

article

09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

9.2K Views

article

10:49

The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

10.9K Views

article

11:48

Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System

24.5K Views

article

09:30

A Guide to Generating and Using hiPSC Derived NPCs for the Study of Neurological Diseases

18.2K Views

article

08:25

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

20.4K Views

article

10:53

Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions

4.6K Views

article

11:42

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

4.8K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved