Transient Transduction of the Strobilated Forms of Echinococcus granulosus

2.8K Views

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13:25 min

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September 16th, 2022

DOI :

10.3791/62783-v

September 16th, 2022

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Mohammad Ali Mohammadi¹, Ali Afgar², Ashkan Faridi³, Seyed Mohammad Mousavi², Ali Derakhshani², Mehdi Borhani⁴, Majid Fasihi Harandi²

¹Student Research Committee, School of Medicine, Kerman University of Medical Sciences, ²Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, ³Department of Parasitology and Mycology, Faculty of Medicine, Kurdistan University of Medical Sciences, ⁴State Key Laboratory for Zoonotic Diseases, Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University

Summary

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Echinococcus Granulosus

Transient Transduction

Lentiviral Vectors

Cystic Echinococcosis

Viral Delivery System

Adult Worm Cultivation

Chapters in this video

0:15

Introduction

1:34

Collecting Hydatid Cysts

3:06

Biphasic Cultivation of Protoscoleces of E. granulosus to Obtain Adult Worms

3:59

Monophasic Cultivation of Protoscoleces of E. granulosus

4:27

Cell Culture for Virus Production and Preparation

5:06

Production and Preparation of the Virus with the Third-Generation Lentiviral Vectors

7:49

Transient Transduction of Different Stages of E. granulosus with the Virus

9:44

Representative Results

11:28

Conclusion

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