The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

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08:50 min

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March 10th, 2023

DOI :

10.3791/64377-v

March 10th, 2023

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Sakshi Choudhary¹, Gefen Livne¹, Shachar Gat¹, Anne Bernheim-Groswasser¹^,²

¹Department of Chemical Engineering, Ben Gurion University of the Negev, ²Department of Chemical Engineering, Ilse Kats Institute for Nanoscale Science and Technology, Ben Gurion University of the Negev

Summary

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Mechanical Properties

Methoxypolyethylene Glycol Maleimide

Chapters in this video

0:05

Introduction

0:59

Cleaning, Hydrophilization, and Silanization

2:30

Surface Passivation with an Inert Polymer

3:20

Preparing Myosin II Motor Aggregates and Actomyosin Solution

4:45

Running an Experiment

5:23

Microscopy Techniques

6:36

Results: Fulfillment of the Poroelasticity Aims

8:27

Conclusion

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