February 10th, 2023
•Here, we describe an immunofluorescence-based method to quantify the levels of single-stranded DNA in cells. This efficient and reproducible method can be utilized to examine replication stress, a common feature in several ovarian cancers. Additionally, this assay is compatible with an automated analysis pipeline, which further increases its efficiency.
Tags
Related Videos
The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics
Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
Evaluating In Vitro DNA Damage Using Comet Assay
Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH
Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle
Generation and Culturing of High-Grade Serous Ovarian Cancer Patient-Derived Organoids
Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved