Visualizing Aip1p-GFP Protein Movement Using Total Internal Reflection Fluorescence Microscopy
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Results: Quantification of Fluorescently Tagged Aip1p Movement in Wild Type and Mutant Cells
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Conclusion
Disease-causing mutations in actin can alter cytoskeletal function. Cytoskeletal dynamics are quantified through imaging of fluorescently tagged proteins using total internal fluorescence microscopy. As an example, the cytoskeletal protein, Aip1p, has altered localization and movement in cells expressing the mutant actin isoform, R256H.
