July 30th, 2014
•Disease-causing mutations in actin can alter cytoskeletal function. Cytoskeletal dynamics are quantified through imaging of fluorescently tagged proteins using total internal fluorescence microscopy. As an example, the cytoskeletal protein, Aip1p, has altered localization and movement in cells expressing the mutant actin isoform, R256H.
Related Videos
Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging
Photobleaching Assays (FRAP & FLIP) to Measure Chromatin Protein Dynamics in Living Embryonic Stem Cells
Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin
Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids
High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells
Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)
Copyright © 2024 MyJoVE Corporation. 판권 소유