Adeno-Associated Virus (AAV) Preparation and Intravitreal Injection of Neonatal Mice
3:13
Retinal Dissections for Live-Imaging Experiment
5:04
Retinal Flat-Mount Preparation
6:35
Time-Lapse Confocal Imaging of Live Whole-Mount Retina Preparations
8:33
Image Deconvolution and Post-Processing in ImageJ
11:16
Results: Representative Results After Neonatal Intraocular Injection, Time-Lapse Imaging, and Deconvolution
12:13
Conclusion
Transkript
Single-cell labeling and visualization of dendritic or axonal arbors is required to study neuronal morphogenesis. By labeling developing arbors in a minimally-invasive manner, retinal tissue remains healthy and suitable for prolonged confocal live
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Here, we present a method for investigating neurite morphogenesis in postnatal mouse retinal explants by time-lapse confocal microscopy. We describe an approach for sparse labeling and acquisition of retinal cell types and their fine processes using recombinant adeno-associated virus vectors that express membrane-targeted fluorescent proteins in a Cre-dependent manner.