This is an alternate and easy to use method for delivering genes of interest to fertilized mouse eggs via laser perforation of the zona pellucida and lentiviral gene delivery. Laser-assisted perforation of the mouse donor procedure may also be app
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Mouse fertilized eggs and early stage embryos are protected by the zona pellucida, a glycoprotein matrix that forms a barrier against gene delivery. This article describes a protocol for perforating the zona with a laser to transduce embryonic cells with lentiviral vectors and to create transgenic mice.