S'identifier

JoVE Journal

Immunology and Infection

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A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis

7.5K Views

08:42 min

April 10th, 2019

April 10th, 2019

7,537 Views

0:04

Title

1:25

Crypt Isolation and Plating from Whole Tissue

5:21

Induction of Experimental NEC and Preparation for Paraffin Embedding

7:22

Results: Analysis of Enteroids

8:21

Conclusion

Transcription

Enteroids are three dimensional organoids derived from intestinal epithelial cells. They are ideal for the study of complex physiological interactions, cell signaling, and host pathogen defense. Our protocol describes how to culture human enteroid

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Enteroids are emerging as a novel model in the study of human disease. The protocol describes how to simulate an enteroid model of human necrotizing enterocolitis using lipopolysaccharide (LPS) treatment of enteroids generated from neonatal tissue. Collected enteroids demonstrate inflammatory changes akin to those seen in human necrotizing enterocolitis.

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